Susceptibility Tests on Insecticides Used to Control Mosquitoes in Jordan

Insecticide susceptibility tests were conducted on five mosquito species collected from two localities in the Jordan Valley (Deir Alla and Ghor as Safi) in 2014. Diagnostic concentration of Temephos (0.25mg/L) was used against the larvae, while diagnostic concentrations of Deltamethrin (0.025%) and Lambdacyhalothrin (0.03g/m2) were used against the adults. The procedures of WHO were followed. A total of 25 larvae were used for adult or larval test for each replicate. All experiments were conducted at 27±2°C. Results showed that all larvae of the tested species in both locations were susceptible to Temephos. The Deir Alla population of An. superpictus adults showed a transit susceptibility (96% pooled mortality) to Deltamethrin. The Ghor as Safi populations of Cx. theirelli and Cx. laticinctus showed resistance to Deltamethrin with 87 and 86% pooled mortality, respectively. Cx. pipiens was resistant to Lambdacyhalothrin at the diagnostic doses (78% pooled corrected mortality). The least pooled corrected mortality (76%), among all populations and species, was found in Deir Alla population of Cx. theirelli, when treated with Lambdacyhalothrin. Further tests are needed to study the response of a larger number of populations covering different locations in Jordan. A control program should be prepared taking in consideration the resistance to insecticide by alternation with different groups including Bti or juvenile hormones.